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1.
Indian J Public Health ; 67(1): 84-91, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37039211

RESUMO

Background: Improved longevity of people living with HIV on highly active antiretroviral therapy and accelerated aging processes are considered contributory to Metabolic Syndrome. Objectives: The current study investigated metabolic syndrome (MetS) in people living with HIV (PLH) who were receiving antiretroviral therapy (ART) under the ongoing National AIDS Control Program. Methods: Clinic attendees (n = 3088) who were on ART for more than 6 months constituted the sampling frame, from which 378 study participants were randomly drawn and included in the analysis following the eligibility check. One hundred and fifty-nine clinic attendees, initiated on ART in ≤6 months, provided an opportunity to estimate the prevalence of MetS in them. Sixty-two PLH from this smaller group were enrolled. Results: MetS was found among 19% (73/378; 95% confidence interval [CI] 15.5%-23.7%) PLH who were on ART >6 months compared with 24% (15/62; 95% CI 14.2%-36.7%) in those who were on ART for ≤6 months based on harmonization criteria for the Asian population; the confidence intervals overlapped and apparently observed difference was not statistically significant. Adjusted for age, body mass index (BMI), protease inhibitor (PI)-based ART regimen, duration of ART, insulin resistance (IR), reported family history of hypertension and residential setting, factors independently associated with MetS were PI containing ART regimen, IR, duration of ART intake and BMI. In the adjusted model, the odds of MetS were three times higher among PLH on PI containing ART regimen (95% CI of adjusted odds ratio; aOR 1.27-8.51) and those having IR (95% CI of aOR 1.48-5.07). The odds of MetS among PLH with BMI ≥23 kg/m2 was 4 (95% CI of aOR 2.08-6.81) times higher than those with lower BMI. Conclusions: MetS in PLH requires the attention of health-care workers in India. Appropriate screening would help initiate early management.


Assuntos
Infecções por HIV , Síndrome Metabólica , Humanos , Síndrome Metabólica/epidemiologia , Estudos Transversais , Índia/epidemiologia , Infecções por HIV/tratamento farmacológico , Infecções por HIV/epidemiologia , Infecções por HIV/complicações , Terapia Antirretroviral de Alta Atividade
2.
J Occup Environ Med ; 65(6): e435-e439, 2023 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-36977361

RESUMO

OBJECTIVE: The aim of the study is to objectively assess if firefighters are meeting the National Fire Protection Association (NFPA) cardiorespiratory fitness (CRF) and American College of Sports Medicine/American Heart Association physical activity (PA) guidelines. METHODS: Two independent fire departments from the Midwest participated in the study. Firefighters wore an accelerometer to track PA and associated intensities. In addition, firefighters completed a stage-graded exercise test TO determine their maximal oxygen uptake (V˙O 2max ). RESULTS: A total of 43 career firefighters completed the study (fire department 1 [FD1]: n = 29, FD2: n = 14). Almost half (44.8% FD1 and 42.9% FD2) met the NFPA CRF guidelines. Compared with the American College of Sports Medicine PA Guidelines of 30 min/d of moderate-to-vigorous PA, more than half of FD2 (57.1%) met the recommended amount of PA, whereas FD1 had less than half (48.3%). CONCLUSIONS: These data demonstrate the need to improve firefighters' PA levels, CRF, and overall health.


Assuntos
Aptidão Cardiorrespiratória , Bombeiros , Humanos , Exercício Físico , Teste de Esforço , Aptidão Física
3.
J Occup Environ Med ; 65(1): 29-33, 2023 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-35902363

RESUMO

OBJECTIVE: The aim of the study is to validate a customized V˙O 2max Graded Exercise Test (GXT) protocol specifically to accommodate firefighters with different cardiovascular fitness levels. METHODS: Career male firefighters (N = 15) completed 3 customized GXTs on a treadmill: 1 in athletic clothes and 2 in their bunker gear to determine maximal oxygen uptake (V˙O 2max ). RESULTS: The on-duty task protocol was reliable, V˙O 2max values of 40.2 ± 4.6 mL·kg·min -1 and 40.3 ± 5.3 mL·kg·min -1 between trials yielded an interclass correlation of 0.911 with a typical error of 1.48 mL·kg·min -1 and a coefficient of variation of 4.0%. The validity analysis indicated consistent maximal V˙O 2 values for the GXTs yielding mean interclass correlation of 0.94 with typical error of 1.16 mL·kg·min -1 and a coefficient of variation of 2.9%. CONCLUSIONS: The customized GXT for structural firefighters has shown to be a reliable, valid, and applicable method of testing cardiovascular fitness in firefighters.


Assuntos
Teste de Esforço , Bombeiros , Humanos , Masculino , Teste de Esforço/métodos , Consumo de Oxigênio , Exercício Físico , Resistência Física
4.
Adv Health Sci Educ Theory Pract ; 28(1): 223-241, 2023 03.
Artigo em Inglês | MEDLINE | ID: mdl-35980515

RESUMO

Greater diversity in the healthcare workforce has been identified as a critical need in serving an increasingly diverse population. Higher education institutions have been tasked with increasing the number of underrepresented students in the health occupations pipeline to better align with the demographics of the general population and meet the need for a diverse health occupations workforce. This study used the National Science Foundation's National Survey of College Graduates dataset to capture data across time, examining the intersectionality of race, gender, and first-generation status on the salary outcomes of students who earn degrees related to health occupations. Results indicate that the intersecting identities of students who earn a bachelor's degree or higher in the health professions impact salary outcomes. Results of this study have implications for higher education policies that can impact increased diversity in the health occupations workforce pipeline.


Assuntos
Ocupações em Saúde , Salários e Benefícios , Humanos , Pessoal de Saúde , Políticas , Estudantes
5.
Pathogens ; 13(1)2023 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-38251333

RESUMO

Staphylococcus aureus is a significant human pathogen with a formidable propensity for antibiotic resistance. Worldwide, it is the leading cause of skin and soft tissue infections (SSTI), septic arthritis, osteomyelitis, and infective endocarditis originating from both community- and healthcare-associated settings. Although often grouped by methicillin resistance, both methicillin-resistant (MRSA) and methicillin-sensitive (MSSA) strains are known to cause significant pathologies and injuries. Virulence factors and growing resistance to antibiotics play major roles in the pathogenicity of community-associated strains. In our study, we examined the genetic variability and acquired antibiograms of 122 S. aureus clinical isolates from SSTI, blood, and urinary tract infections originating from pediatric patients within the southeast region of Virginia, USA. We identified a suite of clinically relevant virulence factors and evaluated their prevalence within these isolates. Five genes (clfA, spA, sbi, scpA, and vwb) with immune-evasive functions were identified in all isolates. MRSA isolates had a greater propensity to be resistant to more antibiotics as well as significantly more likely to carry several virulence factors compared to MSSA strains. Further, the carriage of various genes was found to vary significantly based on the infection type (SSTI, blood, urine).

6.
AIDS Behav ; 26(11): 3597-3606, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-35900708

RESUMO

A well-documented barrier to voluntary medical male circumcision (VMMC) is financial loss due to the missed opportunity to work while undergoing and recovering from VMMC. We implemented a 2-phased outcome evaluation to explore how enhanced demand creation and financial compensation equivalent to 3 days of missed work influence uptake of VMMC among men at high risk of HIV exposure in Zambia. In Phase 1, we implemented human-centered design-informed interpersonal communication. In Phase 2, financial compensation of ZMW 200 (~ US$17) was added. The proportion of men undergoing circumcision was significantly higher in Phase 2 compared to Phase 1 (38% vs 3%). The cost of demand creation and compensation per client circumcised was $151.54 in Phase 1 and $34.93 in Phase 2. Financial compensation is a cost-effective strategy for increasing VMMC uptake among high-risk men in Zambia, and VMMC programs may consider similar interventions suited to their context.


Assuntos
Circuncisão Masculina , Infecções por HIV , Infecções por HIV/epidemiologia , Infecções por HIV/prevenção & controle , Humanos , Masculino , Avaliação de Programas e Projetos de Saúde , Programas Voluntários , Zâmbia/epidemiologia
7.
Public Health ; 202: 93-99, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34933205

RESUMO

OBJECTIVES: The Government of India prohibited the sale of tobacco products during the COVID-19 lockdown to prevent the spread of the SARS-CoV-2 virus. This study assessed the tobacco cessation behaviour and its predictors among adult tobacco users during the initial COVID-19 lockdown period in India. METHODS: A cross-sectional study was conducted with 801 adult tobacco users (both smoking and smokeless tobacco) in two urban metropolitan cities of India over a 2-month period (July to August 2020). The study assessed complete tobacco cessation and quit attempts during the lockdown period. Logistic and negative binomial regression models were used to study the correlates of tobacco cessation and quit attempts, respectively. RESULTS: In total, 90 (11.3%) tobacco users reported that they had quit using tobacco after the COVID-19 lockdown period. Overall, a median of two quit attempts (interquartile range 0-6) was made by tobacco users. Participants with good knowledge on the harmful effects of tobacco use and COVID-19 were significantly more likely to quit tobacco use (odds ratio [OR] 2.2; 95% confidence interval [CI] 1.2-4.0) and reported more quit attempts (incidence risk ratio 5.7; 95% CI 2.8-11.8) compared to those with poor knowledge. Participants who had access to tobacco products were less likely to quit tobacco use compared to those who had no access (OR 0.3; 95% CI 0.2-0.5]. CONCLUSIONS: Access restrictions and correct knowledge on the harmful effects of tobacco use and COVID-19 can play an important role in creating a conducive environment for tobacco cessation among users.


Assuntos
COVID-19 , Abandono do Hábito de Fumar , Abandono do Uso de Tabaco , Adulto , Controle de Doenças Transmissíveis , Estudos Transversais , Humanos , Índia , SARS-CoV-2
8.
Natl Med J India ; 34(3): 138-142, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34825536

RESUMO

Background Paraquat is an inexpensive herbicide used in agriculture because it is easily available and the cost of labour for manual clearance of weeds is prohibitive. Paraquat is toxic to human beings and is also used for committing suicide. We studied the reasons for under-reporting of paraquat poisoning including those related to the training of doctors. Methods In this mixed-methods study, we describe a series of patients with paraquat poisoning. We recorded their demographic data, clinical features, treatment and outcome with an intention to explore the reason for an initial misdiagnosis. We also explored whether deficiencies in curricula contributed to the misdiagnosis. Results The patients of paraquat poisoning (n=28) were mostly young illiterate men driven by impulsive behaviour rather than chronic depression. Paraquat was consumed by patients from non-agricultural background as well, implying easy access to the poison. Many patients could not name the agent and so initial treatment was directed at organophosphorus poisoning. The diagnostic signs included paraquat tongue, renal failure and jaundice. Most of the casualty medical officers and residents were unfamiliar with the symptoms and signs of paraquat poisoning as was evident by their answers to the questionnaire. Knowledge of medical students about paraquat poisoning was not assessed in the theory examinations and viva. Conclusion Factors contributing to the limitation in establishing the diagnosis are illiteracy and ignorance of the patients, lack of specific signs and lack of training of medical officers in treating patients with paraquat poisoning.


Assuntos
Herbicidas , Intoxicação por Organofosfatos , Suicídio , Humanos , Índia/epidemiologia , Masculino , Paraquat
9.
Ann Maxillofac Surg ; 11(1): 160-163, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34522675

RESUMO

RATIONALE: Presenting a rare case report of a giant multilobular lipoma in submandibular and submental spaces of anterior and lateral aspect of neck. PATIENT'S CONCERNS: The patient's main concern was persistent diffuse swelling in right lower face and neck region for 5-6 years. DIAGNOSIS: The lesion was diagnosed as multiple septate lipoma measuring 11.5 cm × 10.5 cm × 6.5 cm involving submandibular region and anterior triangle of the right neck following fine-needle aspiration cytology and radiological imaging. TREATMENT: Extraorally complete surgical excision was carried out through submandibular approach under general anesthesia. OUTCOMES: The patient's postoperative recovery was uneventful. The patient was followed up on a monthly basis for 6 months. No recurrence was observed. The patient was satisfied with the treatment. LESSONS: Lipomas should be considered as a rare differential diagnosis for anterior neck swelling. Biopsy is not necessary to confirm the diagnosis. Surgical excision remains the mainstay of treatment following final diagnosis through imaging modalities.

10.
Front Endocrinol (Lausanne) ; 12: 619176, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33912132

RESUMO

Background: Vitamins B12 and folate participate in the one-carbon metabolism cycle and hence regulate fetal growth. Though vitamin B12 deficiency is widely prevalent, the current public health policy in India is to supplement only iron and folic acid for the prevention of anaemia. Prompted by our research findings of the importance of maternal vitamin B12 status for a healthy pregnancy, birth and offspring health outcomes, we evaluated available literature evidence using a systematic review approach, to inform policy. Methods: A systematic search was performed for relevant Indian studies in the MEDLINE/PubMed and IndMed databases. We selected studies reporting maternal vitamin B12 status (dietary intake or blood concentrations), and/or metabolic markers of vitamin B12 deficiency (homocysteine, methylmalonic acid) or haematological indices during pregnancy and their associations with outcomes of pregnancy, infancy or in later life. Intervention trials of vitamin B12 during pregnancy were also included. Quality of evidence was assessed on the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) system. We followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Results: Of the 635 articles identified, 46 studies met the inclusion criteria (cohort studies-26, case-control studies-13, RCT's -7). There is a high prevalence of vitamin B12 deficiency in Indian women during pregnancy (40-70%) (3 studies). Observational studies support associations (adjusted for potential sociodemographic confounders, maternal body size, postnatal factors) of lower maternal B12, higher homocysteine or an imbalance between vitamin B12-folate status with a higher risk of NTDs (6 studies), pregnancy complications (recurrent pregnancy losses, gestational diabetes, pre-eclampsia) (9 studies), lower birth weight (10 studies) and adverse longer-term health outcomes in the offspring (cognitive functions, adiposity, insulin resistance) (11 studies). Vitamin B12 supplementation (7 RCT's) in pregnancy showed a beneficial effect on offspring neurocognitive development and an effect on birth weight was inconclusive. There is a high quality evidence to support the role of low maternal vitamin B12 in higher risk for NTD and low birth weight and moderate-quality evidence for higher risk of gestational diabetes and later life adverse health outcomes (cognitive functions, risk for diabetes) in offspring. Conclusion: In the Indian population low maternal vitaminB12 status, is associated with adverse maternal and child health outcomes. The level of evidence supports adding vitamin B12 to existing nutritional programs in India for extended benefits on outcomes in pregnancy and offspring health besides control of anaemia. Systematic Review Registration: [website], identifier [registration number].


Assuntos
Ácido Fólico/sangue , Vitamina B 12/sangue , Feminino , Humanos , Índia , Gravidez , Resultado da Gravidez
11.
Indian J Med Res ; 153(3): 241-243, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33906983

Assuntos
Saúde Única , Humanos
13.
Indian J Med Res ; 153(3): 375-381, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33907001

RESUMO

Gorakhpur division consisting of Gorakhpur and neighboring districts Deoria, Kushinagar and Maharajganj in Uttar Pradesh, India, have been witnessing seasonal outbreaks of acute encephalitis syndrome (AES) among children for the last three decades. Investigations conducted during 2005 identified Japanese encephalitis (JE) virus as an aetiology of AES. With the introduction of JE vaccination and other control strategies, the incidence of JE in the region declined, however, outbreaks of acute febrile illness with neurological manifestations continued to occur. Subsequent investigations identified Orientia tsutsugamushi, as the major aetiology of AES outbreaks in the region. This review details clinical, epidemiological, animal and entomological investigations conducted for AES due to O. tsutsugamushi during 2015 and 2017 in Gorakhpur region. Surveillance of acute febrile illness among children attending peripheral health facilities identified scrub typhus as an important aetiology of febrile illness during monsoon and post-monsoon months. Population-based serosurveys indicated high endemicity of scrub typhus. Entomological studies demonstrated natural infection of O. tsutsugamushi in small animal hosts and vector mites. Children acquired this infection through recent exposure to outdoor environment, while playing, or visiting fields or defecating in open fields. A few of the children with scrub typhus progress to develop CNS manifestations. Hence, early administration of appropriate antibiotics is crucial in preventing progression of AFI due to scrub typhus to AES. The investigations conducted by the multi-disciplinary team helped understand the transmission dynamics of scrub typhus in Gorakhpur division and recommend strategies for its control.


Assuntos
Encefalopatia Aguda Febril , Saúde Única , Orientia tsutsugamushi , Tifo por Ácaros , Encefalopatia Aguda Febril/complicações , Encefalopatia Aguda Febril/epidemiologia , Animais , Criança , Surtos de Doenças , Humanos , Índia/epidemiologia , Orientia , Tifo por Ácaros/complicações , Tifo por Ácaros/epidemiologia , Estações do Ano
14.
BMJ Open ; 11(3): e045084, 2021 03 22.
Artigo em Inglês | MEDLINE | ID: mdl-33753443

RESUMO

INTRODUCTION: Fewer than half of all people in the USA have a documented advance care plan (ACP). Hospitalisation offers an opportunity for physicians to initiate ACP conversations. Despite expert recommendations, hospital-based physicians (hospitalists) do not routinely engage in these conversations, reserving them for the critically ill.The objective of this study is to test the effect of a novel behavioural intervention on the incidence of ACP conversations by hospitalists practicing at a stratified random sample of hospitals drawn from 220 US acute care hospitals staffed by a large, nationwide acute care physician practice with an ongoing ACP quality improvement initiative. METHODS AND ANALYSIS: We developed Hopewell Hospitalist, a theory-based adventure video game, to modify physicians' attitudes towards ACP conversations and to increase their motivation for engaging in them. The planned study is a pragmatic stepped-wedge crossover phase III trial, testing the efficacy of Hopewell Hospitalist for increasing ACP conversations. We will randomise 40 hospitals to the month (step) in which they receive the intervention. We aim to recruit 30 hospitalists from up to eight hospitals each step to complete the intervention, playing Hopewell Hospitalist for at least 2 hours. The primary outcome is ACP billing for patients aged 65 and older managed by participating hospitalists. We hypothesise that the intervention will increase ACP billing in the quarter after dissemination, and have 80% power to detect a 1% absolute increase and 99% power to detect a 3.5% absolute increase. ETHICS AND DISSEMINATION: Dartmouth's Committee for the Protection of Human Subjects has approved the study protocol, which is registered on clinicaltrials.gov. We will disseminate the results through manuscripts and the trials website. Hopewell Hospitalist will be made available on the iOS Application Store for download, free of cost, at the conclusion of the trial. TRIAL REGISTRATION NUMBER: NCT04557930.


Assuntos
Planejamento Antecipado de Cuidados , Médicos Hospitalares , Jogos de Vídeo , Idoso , Comunicação , Humanos , Melhoria de Qualidade
15.
Asian Cardiovasc Thorac Ann ; 29(8): 848-851, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33626876

RESUMO

Mycotic pulmonary artery aneurysms requiring pneumonectomy are extremely rare. We present a severely breathless immunocompromised diabetic middle-aged female patient. CT pulmonary angiogram revealed a giant pulmonary artery aneurysm with impending rupture in the right lung. We did an emergency right pneumonectomy under cardiopulmonary bypass support. Histopathology report of the lung specimen confirmed mucormycosis. She received amphotericin B after the procedure. The patient had a prolonged postoperative hospital stay and succumbed to sepsis. Mycotic pulmonary artery aneurysm portends very high morbidity and mortality in immunocompromised patients.


Assuntos
Aneurisma Infectado , Mucormicose , Aneurisma Infectado/diagnóstico por imagem , Aneurisma Infectado/cirurgia , Angiografia , Feminino , Humanos , Pessoa de Meia-Idade , Mucormicose/diagnóstico , Mucormicose/diagnóstico por imagem , Pneumonectomia , Artéria Pulmonar/diagnóstico por imagem , Artéria Pulmonar/cirurgia
16.
Ir J Psychol Med ; 38(2): 145-153, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-32434610

RESUMO

Swift medically led scientifically informed responses to the Covid-19 epidemic nationally have been demonstrably superior to other, non-scientific approaches. In forensic psychiatry and across all psychiatric services, urgent and clinically led responses have underlined redundancies and confusions in the governance of mental health services and a vacuum in policy makers. For the future, a greater emphasis on services for patients with schizophrenia and other severe, enduring mental disorders must aim at reducing standardised mortality ratios, managing risk of violence and improving hard outcomes such as symptomatic remission, functional recovery and forensic recovery of autonomy. This will require more use of information technology at service level and at national level where Scandinavian-style population-based data linkage research must now become legally sanctioned and necessary. A national research and development centre for medical excellence in forensic psychiatry is urgently required and is complimentary to and different from quality management.


Assuntos
COVID-19 , Serviços de Saúde Mental , Esquizofrenia , Psiquiatria Legal , Humanos , SARS-CoV-2
17.
N Engl J Med ; 383(20): 1932-1940, 2020 11 12.
Artigo em Inglês | MEDLINE | ID: mdl-33176083

RESUMO

BACKGROUND: A three-dose, oral rotavirus vaccine (Rotavac) was introduced in the universal immunization program in India in 2016. A prelicensure trial involving 6799 infants was not large enough to detect a small increased risk of intussusception. Postmarketing surveillance data would be useful in assessing whether the risk of intussusception would be similar to the risk seen with different rotavirus vaccines used in other countries. METHODS: We conducted a multicenter, hospital-based, active surveillance study at 27 hospitals in India. Infants meeting the Brighton level 1 criteria of radiologic or surgical confirmation of intussusception were enrolled, and rotavirus vaccination was ascertained by means of vaccination records. The relative incidence (incidence during the risk window vs. all other times) of intussusception among infants 28 to 365 days of age within risk windows of 1 to 7 days, 8 to 21 days, and 1 to 21 days after vaccination was evaluated by means of a self-controlled case-series analysis. For a subgroup of patients, a matched case-control analysis was performed, with matching for age, sex, and location. RESULTS: From April 2016 through June 2019, a total of 970 infants with intussusception were enrolled, and 589 infants who were 28 to 365 days of age were included in the self-controlled case-series analysis. The relative incidence of intussusception after the first dose was 0.83 (95% confidence interval [CI], 0.00 to 3.00) in the 1-to-7-day risk window and 0.35 (95% CI, 0.00 to 1.09) in the 8-to-21-day risk window. Similar results were observed after the second dose (relative incidence, 0.86 [95% CI, 0.20 to 2.15] and 1.23 [95% CI, 0.60 to 2.10] in the respective risk windows) and after the third dose (relative incidence, 1.65 [95% CI, 0.82 to 2.64] and 1.08 [95% CI, 0.69 to 1.73], respectively). No increase in intussusception risk was found in the case-control analysis. CONCLUSIONS: The rotavirus vaccine produced in India that we evaluated was not associated with intussusception in Indian infants. (Funded by the Bill and Melinda Gates Foundation and others.).


Assuntos
Intussuscepção/etiologia , Vacinas contra Rotavirus/efeitos adversos , Administração Oral , Estudos de Casos e Controles , Feminino , Humanos , Imunização Secundária/efeitos adversos , Incidência , Índia/epidemiologia , Lactente , Intussuscepção/epidemiologia , Masculino , Vigilância de Produtos Comercializados , Risco , Infecções por Rotavirus/prevenção & controle , Vacinação , Vacinas Atenuadas/efeitos adversos
18.
BMC Infect Dis ; 20(1): 740, 2020 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-33036575

RESUMO

BACKGROUND: From 2016, the Government of India introduced the oral rotavirus vaccine into the national immunization schedule. Currently, two indigenously developed vaccines (ROTAVAC, Bharat Biotech; ROTASIIL, Serum Institute of India) are included in the Indian immunization program. We report the rotavirus disease burden and the diversity of rotavirus genotypes from 2005 to 2016 in a multi-centric surveillance study before the introduction of vaccines. METHODS: A total of 29,561 stool samples collected from 2005 to 2016 (7 sites during 2005-2009, 3 sites from 2009 to 2012, and 28 sites during 2012-2016) were included in the analysis. Stools were tested for rotavirus antigen using enzyme immunoassay (EIA). Genotyping was performed on 65.8% of the EIA positive samples using reverse transcription- polymerase chain reaction (RT-PCR) to identify the G (VP7) and P (VP4) types. Multinomial logistic regression was used to quantify the odds of detecting genotypes across the surveillance period and in particular age groups. RESULTS: Of the 29,561 samples tested, 10,959 (37.1%) were positive for rotavirus. There was a peak in rotavirus positivity during December to February across all sites. Of the 7215 genotyped samples, G1P[8] (38.7%) was the most common, followed by G2P[4] (12.3%), G9P[4] (5.8%), G12P[6] (4.2%), G9P[8] (4%), and G12P[8] (2.4%). Globally, G9P[4] and G12P[6] are less common genotypes, although these genotypes have been reported from India and few other countries. There was a variation in the geographic and temporal distribution of genotypes, and the emergence or re-emergence of new genotypes such as G3P[8] was seen. Over the surveillance period, there was a decline in the proportion of G2P[4], and an increase in the proportion of G9P[4]. A higher proportion of mixed and partially typed/untyped samples was also seen more in the age group 0-11 months. CONCLUSIONS: This 11 years surveillance highlights the high burden of severe rotavirus gastroenteritis in Indian children < 5 years of age before inclusion of rotavirus vaccines in the national programme. Regional variations in rotavirus epidemiology were seen, including the emergence of G3P[8] in the latter part of the surveillance. Having pre-introduction data is important to track changing epidemiology of rotaviruses, particularly following vaccine introduction.


Assuntos
Gastroenterite/epidemiologia , Genótipo , Hospitalização , Infecções por Rotavirus/epidemiologia , Rotavirus/genética , Doença Aguda , Antígenos Virais/imunologia , Pré-Escolar , Fezes/virologia , Feminino , Gastroenterite/prevenção & controle , Gastroenterite/virologia , Técnicas de Genotipagem , Humanos , Programas de Imunização , Esquemas de Imunização , Técnicas Imunoenzimáticas , Índia/epidemiologia , Lactente , Recém-Nascido , Masculino , Prevalência , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Rotavirus/imunologia , Infecções por Rotavirus/prevenção & controle , Infecções por Rotavirus/virologia , Vacinas contra Rotavirus/imunologia
19.
Indian Pediatr ; 57(12): 1131-1134, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-32893834

RESUMO

OBJECTIVE: To estimate the regional cutoff of optical density (OD) values for immuno-globulin M (IgM) antibodies against Orientia tsutsugamushi in serum and cerebrospinal fluid (CSF) for clinical diagnosis of scrub typhus and immunoglobulin G (IgG) antibodies in serum for sero-epidemiology in Gorakhpur, Uttar Pradesh, India. METHODS: We used data from a serological investigation of acute encephalitis syndrome patients (n=407) during the 2016 outbreak in Gorakhpur, India to determine the cutoff for OD values for IgM antibodies, and from community-based serosurveys (n=1991) to estimate the cutoff for OD values for IgG antibodies. RESULTS: We determined regionally relevant cutoff for OD values of 0.76 for IgM antibodies in serum and 0.22 in cerebrospinal fluid for scrub typhus diagnosis. For serosurveys, IgG antibody cutoff was 1.5. CONCLUSION: We have proposed locally relevant cutoffs for scrub typhus endemic regions, which may be useful for correctly classifying infected population.


Assuntos
Orientia tsutsugamushi , Tifo por Ácaros , Anticorpos Antibacterianos , Humanos , Imunoglobulina G , Imunoglobulina M , Orientia , Tifo por Ácaros/diagnóstico , Tifo por Ácaros/epidemiologia
20.
BMC Ecol ; 20(1): 19, 2020 04 06.
Artigo em Inglês | MEDLINE | ID: mdl-32252741

RESUMO

BACKGROUND: Finger millet is the fourth major crop in Nepal and is cultivated in a traditional integrated subsistence system. Timely rain and appropriate temperature predominately affects crop distribution and yield. Climate change is evident in Nepal and it is imperative to understand how it affects habitat suitability of finger millet. Main objective of this study was to map the current suitable habitat and predicting the potential changes in the future under different climate scenarios in Nepal. Habitat mapping is important for maximizing production and minimizing the loss of local landraces. RESULTS: Maxent model was used in this study to quantify the current suitable habitat and changes in the future habitat suitability of finger millet, based on representative concentration pathways (RCP) (RCP 2.6, 4.5, 6.0 and 8.5) in two different time periods (2050 and 2070AD) using climatic predictive variables and species localities. The model shows that 39.7% (58512.71 km2) area of Nepal is highly suitable for finger millet, with cultivation mostly between 96 and 2300 m above sea level. Eastern and central parts of Nepal have more suitable areas than western parts. Our research clearly shows that the future climatic suitable area of finger millet would shrink by 4.3 to 8.9% in 2050 and 8.9-10.5% under different RCPs by 2070. CONCLUSION: Finger millet is mostly cultivated in mid-hill terraces. The substantial increase in temperature due to climate change may be one reason for decrease in habitat suitability of finger millet. This situation would further threat loss of local landraces of finger millet in the future. The findings can help in planning and policy framing for climate resilient smart agriculture practice.


Assuntos
Eleusine , Agricultura , Mudança Climática , Ecossistema , Nepal
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